Analysis BAP-1 as a Ubiquitin Hydrolase in the BRCA1 Pathway

Abstract

The recurrent theme that has emerged from the structural study of proteins involved in signal transduction and transcriptional regulation is that multiple independently folded globular domains in these proteins often cooperate in macromolecular recognition. These domains are often recognizable by conserved signature amino acid sequence motifs and their spatial organization within a novel protein is often the first clue to its biochemical function. In this aspect, we have identified a novel, nuclear-localized ubiquitin carboxy-terminal hydrolase, BAP-1, that specifically interacts with the RING finger of BRCA1 but not naturally occurring mutants. We are currently investigating the biological consequences of restoring wild-type levels of HP1 in these breast cancer cell lines.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1999
Accession Number
ADA387281

Entities

People

  • David C. Schultz

Organizations

  • Wistar Institute

Tags

DTIC Thesaurus Topics

  • Biomedical And Dental Materials
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Crystal Structure
  • Genetics
  • Health Services
  • Polymer Chemistry
  • Polymeric Films
  • Proteins

Fields of Study

  • Biology
  • Chemistry

Readers

  • Immunology
  • Molecular and Cellular Biochemistry
  • Oncology