Genetic Vaccine Encoding Secreted Glutamic Acid Decarboxylase Prevents Autoimmune Diabetes in NOD Mice
Abstract
Type 1 diabetes results from the destruction of insulin producing beta cells by the immune system. Several immunization methods based on autoantigenic proteins or peptides have been used to prevent autoimmune diabetes in the non-obese diabetic (NOD) mouse. In the work presented here, a gene-based approach was taken for a similar purpose. Intramuscular injection of"naked" plasmid DNA was used to determine the efficacy of genes encoding different versions of a beta cell associated autoantigen for prevention of cyclophosphamide accelerated diabetes in female NOD mice. Four-week old animals received injections of DNA encoding either intracellular or secreted human glutamic acid decarboxylase (GAD), an enzyme found in beta cells and whose recognition by the immune system is thought to be necessary for disease onset.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1999
- Accession Number
- ADA387290
Entities
People
- Alan Escher
- Jingxue Liu
- Maria Filippova
Organizations
- Loma Linda University