A Breast Tumor Suppressor Gene on 8p22: Identification by the Genetic Suppressor Element Approach
Abstract
We are currently investigating a putative tumor suppressor gene (TSG) located at chromosomal band 8p22 that is involved in breast cancer. We have proposed to apply the genetic suppressor element (GSE) approach to the identification of this TSG. Briefly, a library of short gene fragments will be introduced into a cell line which demonstrates suppression of clonogenicity in soft agar with the transfer of chromosome 8. Presumably, the 8p22 TSG is responsible for the suppression of clonogenicity, and the introduction of an "active" GSE from the library into the suppressed cells should inhibit the 8p22 TSU contained in the hybrid cells and allow reversion back to the parental phenotype, the ability to grow in soft agar. Any clones that form will be isolated and further evaluated, as a candidate for the TSU. Currently, several ESTs have been identified in the 8p22 interval using various methods. Construction of the USE library consisting of these ESTs is currently underway. All preliminary optimization experiments for retroviral library delivery have been completed. Any positive USE clones identified will be characterized and evaluated as candidate TSGs.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2000
- Accession Number
- ADA387608
Entities
People
- Kumarika Banerjee
Organizations
- University of Illinois at Chicago