The Molecular Basis of the Response to Radiation
Abstract
In the third year of this CDA Award we have continued to make progress towards all three Technical Objectives. The most challenging problem is the isolation of novel cDNAs encoding human homologs of yeast DNA damage response genes. Major efforts to isolate cDNAs for RAD9 and DUN1 during the previous year have not been successful. In contrast, two hybrid screens have resulted in the isolation of human homologs of RADl8 and RAD2l. Thus, the focus over year 3 has been the characterization of the human Rad2l protein in mammalian cells. We found alterations in expression of human Rad2l mRNA and protein in human breast cancer cell lines. This has lead to development of immunohistochemistry techniques to now expand this research to human breast cancer samples. In Technical Objective 3 we did not see alteration in RAD21 mRNA or Rad2 I protein phosphorylation in human cells exposed to DNA damage. However, we found that induction of the apoptotic pathway (as opposed to DNA damage itself) induces specific cleavage of the human RAD21 cohesin protein. This cleavage product may play a role in signalling subsequent events in apoptosis or result in aneuploidy in cells that survive the apoptotic response.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2000
- Accession Number
- ADA387637
Entities
People
- Sharon Plon
Organizations
- Baylor College of Medicine