Alteration in the Nuclear Structure of Breast Cancer Cells in Response to ECM Signaling
Abstract
Eukaryotic chromosomes are thought to be separated into topologically independent loop domains by periodic attachment onto an intranuclear frame known as the nuclear matrix. Specific DNA sequences that bind to the nuclear matrix are called matrix attachment regions (MARs), in which a specialized DNA context sequences exhibiting high base unpairing propensity (BUR) is typically found. Besides organization of eukaryotic DNA, BURs/MARs may also be important for various functions including replication, transcription and recombination. A strong BUR-binding activity, p1 14, previously reported in breast carcinoma cells, was identified to be a combined property poly ADP-ribose polymerase (PARP) and scaffold attachment factor A (SAF-A). PARP is upregulated in breast carcinoma SK-BR-3 cells as compared to normal mammary epithelial cells in culture. Additionally, HMG 1(Y) was also found to be a BUR-binding protein, and its expression was well correlated with aggressive breast cancer cells. Thus, we have shown that the expression of several BUR-binding proteins correlates strongly with the aggressive phenotype of breast carcinoma cells. Specific binding of these proteins to BURs in cancers may contribute towards gene regulation to trigger or maintain the malignant phenotype in response to signals from extracellular matrix (ECM), or during progression of tumorigenesis and differentiation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2000
- Accession Number
- ADA387652
Entities
People
- Sanjeev Galande
Organizations
- University of California, Berkeley