Tyrosine Kinase Display of Prostate Cancer Cells
Abstract
In this proposal, we present an innovative approach, tyrosine kinase display, to rapidly identify tyrosine kinases differentially expressed in prostate carcinomas. Our hypothesis is that tyrosine kinases play central roles to the growth and progression of prostate carcinomas. The innovative PCR-based approach developed here requires only restriction digestion and gel electrophoresis, and allows all or nearly all expressed tyrosine kinases to be directly "read" from the gel. It is rapid, quantitative, and insensitive to RNA degradation. When fully developed, it is expected to have wide applications in both basic and clinical settings. In the past year (May, 1999 to Apr. 2000) we have made the significant progress, notably 1). We have established comprehensive tyrosine kinase profiles for all five widely used prostate cancer cell lines, as well as those of immortalized prostate epithelial cells and the normal primary cultures of prostate stromal and epithelial cells; 2). We have identified several kinases differentially expressed in prostate cancer cells, in androgen dependent as well as independent cells. These are potential prognostic markers and intervention targets; and 3). We have identified a new kinase whose expression is induced by DHT and which may mediate signals channeled by androgen receptor. Modulation of this kinase activity may restore androgen sensitivity or block androgen independent growth.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2000
- Accession Number
- ADA387675
Entities
People
- Hsing-jien Kung
Organizations
- University of California