DNA Replication Initiator Proteins and Genetic Instability: Creating a Mouse Model for Prostate Cancer
Abstract
We have suggested that limiting hyperploidy by rereplication control may be important for controlling cancer progression. The SCF ubiquitin ligases are a very broad class of enzymes that mediate ubiquitin-dependent destruction of specific proteins. Examples of SCF and related complexes have now been implicated in several tumors including renal cell carcinoma by mutations in the Von Hippel Lindau (VHL) gene. In the fission yeast Schizosaccharomyces pombe, the Pop1 and Pop2 proteins are associated with SCF ubiquitin ligases and are critical for limiting re-replication in yeast. We had identified a close homolog of Pop1 in humans, called hPOP1. Mapping the chromosomal location of the gene by radiation hybrid analysis and FISH, we found the gene to be associated with a region of chromosome 9q34, which has been suggested to contain a tumor suppressor gene for bladder cancer. We sought to explore the connection between human Popi and bladder cancer by looking for alterations in human Popi expression in bladder tumors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA387756
Entities
People
- Peter K Jackson
Organizations
- Stanford University Medical Center