Electrophysiological Changes in NF1
Abstract
The purpose of this study is to examine the inter-relationships between K channels, ras, and neurofibromin in Schwann cell (SC) proliferation. This study will aid our understand of how mutant neurofibromin contributes to the tumorigenic process. Preliminary data on K currents of SC from a cutaneous neurofibroma raise the possibility that neurofibroma SC ion channels are not physiologically abnormal or that the ion channel phenotypes of normal, neurofibroma-derived and neurofibrosarcoma-derived SC represent a continuum that requires closer observation for differences between normal and neurofibroma-derived SC. The neurofibrosarcoma cell line T265 had tumored K currents like those of 3 other neurofibro-sarcoma-derived cell lines. T265 cells have proved useful in beginning experiments to understand the role of K channels in SC proliferation. TEA analogs applied for 41-69 hrs' continuous exposure to T265 cells block K channels, block proliferation, and decrease resting potentials. The link between K channels and proliferation has been suggested for SC and other cell types, but is not clear how this link functions. NFl tumored SC cultures, which span a continuum of non-malignant, non- or slowly-proliferating phenotypes to proliferating, malignant phenotypes, and to which normal cultured SC can be compared, represent a unique and relevant model for understanding the role of K channels in proliferation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2000
- Accession Number
- ADA387882
Entities
People
- Lynne A. Fieber
Organizations
- University of Miami