Estrogens, Microtubules, and Aneuploidy: Mechanisms of Mammary Gland Tumorigenesis
Abstract
The remaining studies for this project were to be performed in primary and secondary cultures of rat mammary epithelial cells (RMEC) prepared from ACI and Sprague-Dawley rats. Eight attempts were made to grow these cells, either from freshly prepared cell suspensions or from cryopreserved cells. None of these attempts were successful. Cells began to exhibit an epithelial morphology but within 24-48 hours they ceased growth and detached from the collagen substratum. None of the cells were acceptable for studies either of estradiol metabolism or of the effects of estrogens on mitotic microtubules. This failure to grow these cells prevented the performance of the remaining studies. We recently have acquired spontaneously immortalized lines of RMEC from the estrogen-sensitive ACI hybrid strain and from the estrogen-resistant Copenhagen strain, one of the parental strains of the ACI. Our plan for the remaining funding period is to pursue our aims related to mitotic microtubules and the induction of aneuploidy using these cell lines. Although they are presumed to no longer express cytochrome P450s, and thus cannot metabolize estradiol itself, we nonetheless can treat these cells with estradiol and various metabolites, particularly the catechol estrogens, to determine the ability of these compounds to alter mitosis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2000
- Accession Number
- ADA387982
Entities
People
- Gregory Reed
Organizations
- University of Kansas Medical Center