Rational Design of Human Prolactin Receptor Antagonists for Breast Cancer Therapy

Abstract

There were three tasks proposed for the first year of this project. All three tasks have been accomplished. We have cloned and confirmed by sequencing the cDNAs encoding hPRL, hPRL-G129R and hPRL-BP (task 1). All three cDNAs have been transfected into mouse L cells and stable mouse L cells have been established (task 2). We have also maintaining 12 human cancer cell lines purchased from ATCC(task 3). In addition, we have demonstrated that hPRL-G129R is able to inhibit breast cancer cell proliferation via induction of apoptosis (Clinical Cancer Research, 5:3583-3593, 1999) . We are confident that the project will go on as proposed in years two and three.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2000
Accession Number
ADA388013

Entities

People

  • Wen Y. Chen

Organizations

  • Clemson University

Tags

DTIC Thesaurus Topics

  • Apoptosis
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Media
  • Culture Techniques
  • Fish
  • Gene Expression
  • Health Services
  • Medical Personnel
  • Neoplasms
  • Tumor Cell Line

Readers

  • Breast cancer cell signaling and growth regulation.
  • Clinical Trial Research.
  • Molecular Biology and Genetics