G1 Cell Cycle Control by Regulated Proteolysis in Normal and Tumorigenic Breast Cells
Abstract
The p53 tumor suppressor gene mediates a major tumor suppression pathway in mammalian cells that is frequently altered in human cancers, including breast cancer. p53 protein is kept at low level during normal cell growth by its short half-life and is stabilized following oncogenic stimulation and DNA damage. Growing evidence has identified oncoprotein MDM2 (mouse double minute) as a key negative regulator and tumor suppressor ARF (alternative reading frame) as a key positive regulator of p53 protein stability. Two findings were made over the past year that extended our understanding into the p53 control by MDM2 and ARF. First, we found that human tumor suppressor ARF impedes S-phase progression independent of p53. Second, we discovered that p53 protein contains two separate nuclear export signals (NES) that functional collaborate to mediate an MDM2-independent nuclear export.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2000
- Accession Number
- ADA388020
Entities
People
- Yue Xiong
Organizations
- University of North Carolina at Chapel Hill