Predicting Response to Adjuvant Therapy With Tamoxifen
Abstract
It is our hypothesis that multiple patterns of cDNA expression characterize the acquired Tam-resistant phenotype, and that these expression profiles can be ascertained using cDNA microarrays. We propose three technical objectives to identify mechanisms and/or the specific gene profiles contributing to acquired Tam resistance. In Aim 1 we will identify Tam resistance expression profiles using models of acquired resistance. This will be accomplished using two models of resistance and commercially available expression arrays. Representative gene candidates will be confirmed for relative expression using Western blot and RT-PCR analyses. In Aim 2 we will profile resistance patterns in clinical breast cancer samples. We will utilize Affymetrix arrays to profile tamoxifen sensitive and tamoxifen resistant tumors from patients. In Aim 3 we will evaluate specific candidate genes for their involvement in the genesis and progression of Tam resistance. We will evaluate the hormone-responsive phenotype of candidate genes in vitro utilizing stable transfection of full-length and antisense cDNAs. Their growth potential, invasive, and metastatic properties will also be examined in vitro.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2000
- Accession Number
- ADA388192
Entities
People
- Suzanne A. Fuqua
Organizations
- Baylor College of Medicine