Role of Accessory Molecular in Endotoxin-Endothellial Interactions and Endothelial Barrier Dysfunction

Abstract

Bacterial lipopolysaccharide (LPS) is a component of Gram-negative bacteria endothelial barrier function. We used an in vitro experimental system in which LPS derived from Escherichia coli 0111:B4 was presented to postconfluent bovine pulmonary artery endothelial cell (EC) monolayers cultured on filter supports mounted in chemotaxis chambers. Transendothelial flux of 14C-bovine serum albumin was used as a quantitative measurement of paracellular permeability. We studied the roles of protein tyrosine phosphorylation and apoptosis in LPS-induced opening of the paracellular pathway and established that the lipid A moiety was the active portion of the LPS molecule in the non CD14-bearing EC system.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1998
Accession Number
ADA388219

Entities

People

  • Simeon E. Goldblum

Organizations

  • University of Maryland School of Medicine

Tags

DTIC Thesaurus Topics

  • Albumins
  • Amino Acids
  • Anti-Bacterial Agents
  • Apoptosis
  • Bacteria
  • Biological Sciences
  • Biomedical And Dental Materials
  • Blood
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Gram-Negative Bacteria
  • Intercellular Junctions
  • Laboratory Animals
  • Molecules
  • Polymeric Films
  • Proteins

Fields of Study

  • Biology
  • Chemistry
  • Medicine

Readers

  • Immunology and Pathology
  • Microbial Pathology
  • Molecular and Cellular Biochemistry