Role of Melatonin in the Prevention of Breast Cancer in Patients With Cystic Breast Disease

Abstract

Melatonin and breast cyst fluids (BCFs) both exert antiproliferative effects on breast cancer cells that may be mediated by growth factors. Our research was the first to identify melatonin in BCF and we hypothesize that it is responsible for BCF's antiproliferative properties. The primary objectives of this study are to establish a clinical BCF sample bank among patients with gross cystic breast disease in order to study the relationship between this disease, melatonin and related growth agents in BCF, and breast cancer risk. A laboratory effort will use BCF samples to elucidate the contribution of melatonin and related growth agents (EGP, TGF-beta, DHEA-S) to the oncostatic effects of BCFs in the MCF-7 human breast cancer cell model. Our progress to date includes the establishment of informed consent procedures and a BCF sample bank. Patient enrollment and BCF sample collection are on-going. Cell culture experiments have confirmed that physiological melatonin levels inhibit MCF-7 cell growth. We have also performed baseline melatonin, sodium, potassium, and TGF-beta measurements in BCF. Out of 17 subtasks specified in our Statement of Work, 8 have either been completed or were initiated in year 1 and will remain on-going as patient enrollment progresses.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2000
Accession Number
ADA388220

Entities

People

  • Robert L. Wells

Organizations

  • Colorado State University

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Techniques
  • Growth Factors
  • Health Services
  • Medical Personnel
  • Peptide Growth Factors
  • Radioactive Materials
  • Tumor Cell Line

Fields of Study

  • Medicine

Readers

  • Agricultural Chemistry/Soil Science
  • Oncology (Cancer Research).
  • Oncology and Biomarker-Based Cancer Detection.