Fluorescence Spectra of Individual Flowing Airborne Biological Particles Measured in Real Time
Abstract
The UV-excited fluorescence spectra of individual flowing biological aerosol particles as small as 2 pm in diameter have been measured in real time (rates up to 10 particles per second). The particles are illuminated with a single shot from a Q-switched 266- or 351-nm laser. The signal-to-noise ratio and resolution of the spectra are sufficient for observing small line-shape differences among various types of bioaerosols (e.g., bacteria versus pollens) and between the same types of bioaerosol prepared under different conditions (e.g., the unstarved and four-month-starved E. coli strain DH5). Multiple-wavelength excitation provides additional information for distinguishing bioaerosols based on their fluorescence spectra.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2001
- Accession Number
- ADA388322
Entities
People
- Ronald G. Pinnick
- Stanley Niles
- Stephen Holler
- Steven C. Hill
- Yong-Le Pan
Organizations
- United States Army Research Laboratory