Development and Use of Novel Tools to Directly Screen for Substrates of Cyclin Dependent Kinases
Abstract
The aim of this research is to gain further insights into the means by which cell proliferation is controlled by cyclin dependent kinases. To this end, a screen has been carried out for proteins that interact with the budding yeast cdk, Cdc28. 10 novel interacting proteins have been identified in a two-hybrid screen using a Gal4/Cdc28 fusion as the bait. Several of the putative interacting proteins have been shown to interact with specific cyclin-Cdc28 kinase complexes. For each of the 10 two-hybrid positives the nature of the Cdc28 interaction is currently being determined by biochemical means. The functional difference between cyclins in their abilities to induce and repress transcription has been investigated using the genomic technology of microarrays. In addition, the complete set of cell cycle regulated genes in yeast has been defined. This work has now been published (MBC 9:3273-3297 1998), and a full description and complete data sets are available on a public website at http://cellcycle-www.stanford.edu.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2000
- Accession Number
- ADA388443
Entities
People
- Joseph Donovan
Organizations
- Cold Spring Harbor Laboratory