Insecticide Exposure in Parkinsonism
Abstract
Behavioral, neurochemical, and immunocytochemical studies characterized the possible role of insecticide exposure in the etiology of Parkinson's disease as it may relate to Gulf War Syndrome. Chlorpyrifos (CP) and permethrin (PM) were given 3 times over a two week period by injection (CP, subcutaneous and PM intraperitoneal). PM (1.5 mg/kg) increased dopamine transport 33%, which is at least two orders of magnitude below its LD5O. CP (100 mg/kg) reduced transport 10% compared to controls. Doses of PM greater than or equal to 25 mg/kg lowered transport activity significantly below that of control. Toxic effects may have been involved in reduced transport, since 3-Hgbr12935 binding was unaffected, suggesting no change in transporter density. Both PM and CP caused small, but statistically significant decreases in mitochondrial dehydrogenase activity. However, cytotoxicity was not reflected in reduced levels of striatal dopamine or tyrosine hydroxylase staining, which were not changed by high doses of CP or PM. Both insecticides significantly potentiated the dopamine-depleting action of MPTP. But, effects on dopamine titers in combination treatments were not accompanied by changes in behavioral assays. Insecticides also showed differential regulation of muscarinic receptor density in the striatum, where PM up-regulated receptors at doses >25 mg/kg, and CP down-regulated receptors at 100 mg/kg.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2001
- Accession Number
- ADA388559
Entities
People
- Jeffrey Bloomquist
Organizations
- Virginia Tech