Molecular Determinants of Cellular Sensitivity to Flavopiridol, and Anti-Cell Signaling Anticancer Agent

Abstract

Flavopiridol is an investigational drug undergoing Phase II clinical trials for the treatment of various solid tumors. This drug has been shown to inhibit of the cyclin-dependent protein kinases, which are essential mediators of the cell division cycle. While this activity is likely to be responsible for its anticancer activity, it is nevertheless unclear how this drug achieves selective toxicity. To address this question, we created a flavopiridol-resistant cell line. The experiments described in this proposal are designed to identify the mechanism(s) responsible for rendering this cell line drug-resistant. Levels of cyclin-dependent protein kinase activity in this resistant cell line will be determined, and compared to those seen in the parental, drug-sensitive cell.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2000
Accession Number
ADA388574

Entities

People

  • Colin R. Campbell

Organizations

  • University of Minnesota

Tags

DTIC Thesaurus Topics

  • Antineoplastic Agents
  • Biomedical Research
  • Breast Cancer
  • Cell Division
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Clinical Trials
  • Culture Techniques
  • Cytotoxins
  • Drug Resistance
  • Electronic Mail
  • Neoplasms
  • Proteins
  • Resistance
  • Sensitivity
  • Toxicity

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Oncology (Cancer Research).