Identification of Surrogate Markers for Estrogen Receptor Action in Breast Cancer Cells
Abstract
Recent studies have described the identification of novel estrogen and antiestrogen inducible genes with interesting paradigms of regulation. However, due to the paucity of known estrogen receptor (ER) genomic targets, it is still not clear how estrogen displays mitogenic effects in the breast, and how these processes are dysregulated during the onset of hormone resistance. It is clear that the discovery of novel estrogen regulated genes will enable us to better define the key regulators of growth in ER-positive breast cancer cells. In the current study, differential display PCR was used to identify surrogate markers of estrogen and antiestrogen action in human breast cancer cells. A novel transcript was discovered that is upregulated by both the ER agonist l7beta-estradiol and the antagonist ICI l82,78O. The full length cDNA was cloned and identified as hMIP, the human homologue of the mitochondrial intermediate peptidase (hMIP), an enzyme important in cellular respiration. A second estrogen-responsive cDNA, encoding the control region of the mitochondrial genome, was also discovered. These studies highlight the role of ER pathways in mitochondrial function, and suggest that estrogen-stimulated mitogenesis in breast tissue may involve the enhancement of cellular processes of metabolism and energy generation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2000
- Accession Number
- ADA388636
Entities
People
- David Mcdonnell
- Julianne Hall
Organizations
- Duke University Hospital