Developmental Approach to Characterizing the Invasion Gene Program in Breast Cancer
Abstract
Tile changes in the gene program of neoplastic cells that regulate the expression of an invasive phenotype are largely undefined. Direct comparisons of the gene expression profile displayed in normal and carcinomatous breast tissues have provided insights into the mechanisms underlying tumor progression. However, attempts to identify the gene products differentially expressed during invasion in vivo have been hampered by the fact that only a small percentage of the cells recovered from a tumor mass are actively engaged in invasive behavior at the time of isolation. Because tissue remodeling induced during mammary gland involution bears homology to early stages of carcinogenesis, the involuting mammary gland may be used to identify genes that control matrix turnover in cancerous states. To this end, we propose to (1) generate cDNA libraries from control versus involuting mouse mammary glands, (2) isolate differentially expressed genes during matrix remodeling, (3) identify differentially expressed genes that encode secretory proteins associated with the involution program and iv) identify human homologues of the mouse-derived matrix remodeling genes. Tile approach should allow for the identification of gene products relevant to breast cancer invasion.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2000
- Accession Number
- ADA388915
Entities
People
- Stephen J. Weiss
Organizations
- University of Michigan