Cripto-1 in Mammary Gland Development and Carcinogenesis
Abstract
The purpose of this study is to evaluate the role of the growth factor Cripto-l (CR-1) in mammary development and carcinogenesis. During the final year of this grant we developed adenoviral vectors for the expression of CR-1 and cloned CR-3 from MCF-7 cells. We overexpressed CR-1 and CR-3 in MCF-7, Vero, and HC-11 cells, and found a dramatic difference in phenotype of the transduced cells. This may indicate that the few differences present between CR-1 and CR-3 are in functional regions of the protein. We are currently developing GFP-free adenoviral vectors and viruses that only contain one of the differences between CR-1 and CR-3 to define which of the amino acid differences are responsible for the difference in phenotype of transduced cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2000
- Accession Number
- ADA388939
Entities
People
- H. L. Hosick
- Randall L. Kinter
Organizations
- Washington State University