BRCA2 and the DNA Double-Strand Break Repair Machinery
Abstract
The overall goal of my grant proposal is to test the model, BRCA2 modulates the early steps of repair mediated by the Rad5O nuclease complex, and the later stages catalyzed by the Rad51 recombinase. To test our hypothesis, we proposed to accomplish the following specific aims. Aim1. To establish the specificity of the interaction between Rad5O, RINT-1 and BRCA2. Aim2. To determine how BRCA2 influences double strand break repair machinery. We found that human RINT-1 shares sequence homology with a novel protein identified in Drosophila melanogaster, including a coiled-coil domain within its N-terminal 150 amino acids, a conserved central domain of about 350 amino acids, and an C-terminal region of 90 amino acids exhibiting 35-38% identity. While Rad5O and RINT-1 are both expressed throughout the cell cycle, RINT-1 specifically binds to Rad5O only during late S and G2/M phases, suggesting that RINT-I may be involved in cell cycle regulation. The characterization of RINT-1 protein represents an important step toward our goal in uncovering the significance of the interaction between BRCA2, RINT-1 and Rad5O nuclease complex.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2000
- Accession Number
- ADA388959
Entities
People
- Phang-lang Chen
Organizations
- University of Texas Health Science Center at San Antonio