Induction of Apoptosis in Human Breast Cancer by Adenoviral-Mediated Gene Transfer of the Transcription Factor E2F-1
Abstract
We have previously shown that the transcription factor E2F-I results in apoptotic cell death in breast cancer cells when overexpressed in using an adenoviral-mediated system. In order to determine whether the E2F-1 transgene is effective in sensitizing cells to chemotherapy-induced apoptosis, we utilized a recombinant adenovirus in combination with Taxol or doxorubicin and evaluated our results using a two- dimensional isobologram statistical analysis. We observed marked synergistic growth inhibitory effects in breast cancer cell lines treated with a low-dose of adenovirus E2F-1 and low doses of Taxol or doxorubicin. In conclusion, adenovirus-mediated expression of E2F-1 can inhibit breast cancer cell growth synergistically in combination with low-dose Taxol. This combination of gene therapy and chemotherapy may lower the dose of chemotherapeutic agents necessary in the treatment of breast cancer patients and thus may reduce the adverse effects seen with chemotherapy treatments. We plan to explore this further in an animal model using breast cancer xenografts in nude mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2000
- Accession Number
- ADA389230
Entities
People
- Kelly Hunt
Organizations
- The University of Texas MD Anderson Cancer Center