The Development of BRCA2-Deficient Mice
Abstract
Women who inherit a BRCAl or BRCA2 mutation have an 80-90% chance of developing breast cancer. Gene targeting techniques were used to create a Brca2- deficient mouse. A portion of exon 10 was replaced with the Neo gene resulting in premature truncation of the protein product. Examination of normal mammary ductal development in l29(+/+) and 129(+/Brca2-) mice revealed phenotypic differences between the two genotypic classes. l29(+/+) and 129(+/Brca2-) mice and several inbred mouse strains were used to study the effect of genetic background on radiation induced mammary tumor induction. BALB/c and SWR mice are susceptible to radiation induced mammary tumorigenesis and c57BL/6, FVB/N and C3H mice are relatively resistant. While FVB/N mice do not develop tumors, radiation treatment has a dramatic effect on ductal morphogenesis. To date, no mammary tumors have been observed in the 129(+/+) and 129(+/Brca2 -) mice. Brca2-deficient mice were crossed to p53 mutant mice to generate four genotypic classes of offspring. A study is in progress to assess the consequences of harboring mutation in the two tumor suppressor genes with and without radiation exposure. Mouse strains more and less sensitive to mammary tumor induction are being used to make mice congenic for the Brca2 mutation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2000
- Accession Number
- ADA389252
Entities
People
- L. M. Bennett
- R. Wiseman
Organizations
- National Institute of Environmental Health Sciences