Analysis of HRAD1, a Human G2 Checkpoint Control Gene

Abstract

Genomic instability has long been thought to contribute in tumorigenesis. Cell cycle checkpoints are a key regulatory mechanism, which maintains the integrity of the genome. The S-Phase and G2/M checkpoints have been shown to halt cell cycle progression in response to aberrant DNA structures during replication as well as in response to DNA damaging agents. The G2/M checkpoint gene hRadl has been demonstrated to be essential for checkpoint activity in humans. HRadl has been demonstrated by our lab to exist in a ternary complex with two other checkpoint proteins, hRad9 and hHusl. Since taking over the project in November of 1999, I have been pursuing other interacting partners with the hRadl, hRad9, hHusl complex by various methodologies, including: Yeast-2-Hybrid, Gel Filtration chromatography, and metabolic labeling.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2000
Accession Number
ADA389268

Entities

People

  • Blair Besley
  • Scott Davey

Organizations

  • Queen's University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Eukaryotes
  • Filtration
  • Fungi
  • Genetic Structures
  • Genomic Instability
  • Hybrid Systems
  • Materials
  • Metabolic Diseases
  • Molecular Biology
  • Neoplasms
  • Proteins
  • Recombinant Dna
  • Skin Diseases

Fields of Study

  • Biology

Readers

  • Applied Combinatorial Optimization and Logic Circuit Design.
  • Molecular Biology and Genetics
  • Molecular Genetics