Development of Rapid in Vitro and In Vivo Assays to Detect and Quantify MYC Network Protein Associations
Abstract
Members of the myc oncoprotein family contribute to the pathogenesis of many human malignancies. Myc proteins are members of the bHLH-ZIP family of transcription factors that bind to DNA in heterodimeric association with another bHLH-ZIP protein, max. We originally proposed to develop an in vivo assay for myc-max association using green fluorescent protein (GFP)-tagged molecules. We suggested that formation of myc-max heterodimers might be associated with quantifiable fluorescence resonance energy transfer (FRET) and that loss of the FRET signal could be used as a way of screening for drugs that disrupt myc-max association. Although we have been unsuccessful in this regard, we have been quite successful in developing a highly sensitive yeast-based assay. Current efforts are focused on adapting this assay into a 96 well plate format that can rapidly and reproducibly be used for the screening of several low molecular weight compound libraries available to us. Over the next year, we hope to use this screening procedure to identify candidate drugs that will be further tested for their specificity and sensitivity in promoting myc-max dissociation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2001
- Accession Number
- ADA389277
Entities
People
- Edward V. Prochownick