Butyate-Induced Apoptosis in Prostate Cancer Cell Lines
Abstract
Histone deacetylase inhibitors (HDAI's) are a promising class of experimental therapeutics. While rapid, butyrate-induced apoptosis is less prominent than among other cancers, cells from 4 of the 6 PrCa lines were irreversibly growth inhibited, especially at low population densities. The JCA-1 cell line continued to replicate in the presence of butyrate or SBHA (another HDAI). The other HDAI-tolerant line, TSU-PR1 could almost fully recover replication abilities upon removal of butyrate when treated at high cell densities, but was severely inhibited when treated at colony forming assay densities. The mechanisms responsible for these distinct forms of HDAI tolerance are the subject of continuing investigation. One of the goals of our original proposal was to attempt to establish new cell lines from prostate cancers to achieve a better representation of moderately-well differentiated cancers among available laboratory models. We have been unsuccessful at establishing such lines from prostatectomy-derived tumor tissue, by convention unlikely to represent aggressive disease. Metastatic PrCa cells, obtained by bone marrow biopsy of persons with advanced disease, will be added to dense cultures of normal bone stroma.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2000
- Accession Number
- ADA389280
Entities
People
- John A. Mcbain
Organizations
- Dartmouth College