Modulation of Adhesion Molecule Expression on Prostate Tumor Cells After Co-Culture With Eosinophilic Cell Lines
Abstract
We have demonstrated that cell lines developed from metrizamide density fractions of peripheral blood hypodense (.22) and hyperdense (.24) eosinophils significantly inhibited UNCAP PC3, DUl45 and HPCl monolayer growth cultures in vitro. Thus actvity was enhanced when the eosinophils were pretreated with interleukin-5. DUl45 and PC3 colony formation was inhibited 50-75% by eosinophil cell lines while peripheral blood eosinophils inhibited PC3 colony formation by as much as 95%. 24hr. cultured eosinophil supernatants significantly inhibited PC3 colony formation with 3/7 supernatants causing 100% inhibition. Inhibitory activity of the 24hr supernatants towards DU145 was more variable ranging from 9-90% inhibition of colony formation. There was slight enhancement of growth with one preparation. IL-4 and TNF-alpha, which were found present in the supernatants also inhibited growth of monolayers cultures of PC3, DU145 and HPCl. Flow cytometric analysis of adhesion molecule expression post; eosinophil; tumor cell co-culture, eosinophils 24hr culture supernatant treatment and exogenous cytokine treatment, revealed that 1)1-CAM was upregulated by IL-1, TNF-alpha and IL-lO, on DU145 and PC3 and induced on UNCAP. ELAM-1 which was induced on PC3 by TNF-alpha, IFN(y), IL-lO and IL-12, but not IL-4, VCAM-1 was induced by IL-lO only. Most significantly, the suppressor molecule E-cadherin was induced on both DU145 and PC3 by IL-12 which has been found in eosinophil 24hr supernatants. These data strongly support the hypothesis that eosinophils can destroy prostate tumor cells in vitro. The eosinophil cell lines and cytokine modulation of their activity offer an exquisite tool for more detailed study (both cellular and molecular) of a role for eosinophils as anti-prostate cancer effector cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2000
- Accession Number
- ADA389285
Entities
People
- Paulette M. Furbert-harris
Organizations
- Howard University