Conditional Estrogen Receptor Knockout Mouse Model for Studying Mammary Tumorigenesis
Abstract
Towards achieving our overall goal of generating ER knockout mice for studying mammary tumorigenesis, we had proposed to generate ER-alpha conditional knockout mice. Towards this end, we have faced some difficulty in constructing the targeting construct required for the generation of knockout mice. This delay in making the gene targeting construct for ER-alpha gene ablation was mainly due to the personnel problem: Dr. Fan Xu, a postdoctoral fellow on this grant, decided to quit only after working for 4 months. Since his departure, we are waiting for another Research Assistant to join the lab, whose H-1 visa has just been approved by the INS and he expected to start work in my lab on February 1, 2001. In the mean time, as presented in this progress report, we have obtained exciting new data with regard to ER-alpha and ER-beta interaction and their target genes in breast cancer cell lines. Our major findings include the demonstration that ER-beta forms the strongest homodimer followed by ER-alpha/beta heterodimer and ER-alpha homodimer. The second very important finding is the progress made in identifying target genes for ER-alpha in the breast cancer. Knowledge of which genes are modulated by which dimer pairs of estrogen receptors will be of great importance in interpreting our results in the knockout mice, a central theme of our original proposal.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2001
- Accession Number
- ADA389417
Entities
People
- Schaib A. Khan
Organizations
- University of Cincinnati