Nuclear Tubulin: A Novel for Breast Cancer Chemotherapy

Abstract

Our research is based on our observation that the beta x isotype of tubulin is found in the nuclei of breast cancer cells. Our goals are to learn the function of 11 and to design a novel anti-tumor drug that will target beta x. We have found that beta x enters the nucleus without going through the nuclear membrane. Instead, beta x binds to a nuclear component after the nucleus has disintegrated during mitosis and then remains in the nucleus when the nucleus re-forms after mitosis. The process by which beta iv is localized to the nucleus is specific for beta x; neither beta x nor beta iv do this. It is possible that the nuclear component to which (3"binds could be a novel target for anti-tumor drugs. We have found that vinbiastine and taxol, two well-known anti-tumor drugs, cause (beta x to be expelled from the nucleus. This raises the possibility that this may be part of their mechanism of action. We have also synthesized a derivative of colchicine bound to a peptide with a nuclear localization sequence; this peptide is likely to target nuclear tubulin.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2000
Accession Number
ADA389423

Entities

People

  • Richard Luduena

Organizations

  • University of Texas Health Science Center at San Antonio

Tags

DTIC Thesaurus Topics

  • Alkaloids
  • Breast Cancer
  • Cells
  • Chemistry
  • Chemotherapy
  • Confocal Microscopy
  • Connective Tissue Cells
  • Cytoplasm
  • Cytoskeleton
  • Ionizing Radiation
  • Mass Spectrometry
  • Materials
  • Microscopy
  • Neoplasms
  • Proteins
  • Radiation
  • Tumor Cell Line

Fields of Study

  • Biology
  • Physics

Readers

  • Molecular Biology and Genetics
  • Parasitology and Pharmacology of Malaria.
  • Quantum spin resonance or Electron Paramagnetic Resonance spectroscopy.