New Gene Based Probes for Imaging Breast Cancer with PET

Abstract

We are developing methods to image her-2-neu oncogene over-expression in breast cancer using positron emission tomography (PET) . Small oligodeoxynucleotides (ODNs) that are complementary to the Her-2-neu messenger RNA (mRNA) are being investigated as potential imaging probes. Fluorine-18 (2 hour half-life positron emitter) has been used to label 15-18 mer ODN probes. The labeling of an ODN to Fluorine-18 has been particularly troublesome because of the limited half-life and the complicated chemistry. We have explored multiple strategies and are trying to maximize yield and specific activity of our probes. With adequate synthesis of the ODN probes we will begin further cell testing and small animal imaging with microPET. We expect that the techniques developed will lead to methods to detect breast cancer in living subjects in the case that her-2-neu is over-expressed.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2000
Accession Number
ADA389488

Entities

People

  • Sanjiv Gambhir

Organizations

  • University of California, Los Angeles

Tags

DTIC Thesaurus Topics

  • Antisense Elements (Genetics)
  • Biomolecules
  • Breast Cancer
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Deoxyribonucleic Acids
  • Fluorine
  • Health Services
  • Molecules
  • Mrna
  • Neoplasms
  • Positrons
  • Proteins
  • Surface Plasmon Resonance

Fields of Study

  • Physics

Readers

  • Medical Imaging.
  • Molecular Genetics