Identification of Junctionally-Transmitted Growth Inhibitors
Abstract
We have proposed that one function of gap junctions is to transmit growth control signals. There is abundant evidence that most cancers, including breast cancers, lose the capacity to junctionally communicate and we hypothesize that this in part is responsible for their aberrant behavior. To test this hypothesis rigorously we have genetically engineered human breast cancer cells to contain the gap junction gene connexin 43 under the control of the tetracycline-inducible promoter. In the first year of this grant application, we genetically engineered MDA-435 cells to express connexin 43 under the control of a tetracycline-inducible promoter. We demonstrated that on removal of doxycycline, cells rapidly express connexin 43, integrated into the plasma membrane and became junctionally competent.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2000
- Accession Number
- ADA389496
Entities
People
- John Bertram
Organizations
- University of Hawaiʻi System