Genetic Induction of Cytolytic Susceptibility in Breast Cancer Cells
Abstract
These studies continue to focus on defining the mechanisms by which the ElA oncogene sensitizes human tumor cells to destruction by proapoptotic, immunological and chemical injuries. Translation and testing of the key observations from an NIH-3T3 cell system to a human breast cancer cell system has been completed, and an important difference between these systems has been detected - i.e., in contrast to mouse fibroblasts, chemotherapy- induced injury of ElA-positive human tumor cells does not require expression of the wild type p53 gene. This finding has potentially important implications for the clinical relevance of the findings from this work. cDNA array studies of ElA-related differences in cellular gene expression that might be involved in the ElA-induced phenotypic change from apoptosis-resistant to apoptosis-sensitive has been initiated. Preliminary data indicate that some findings in mouse NTH-3T3 cells will be translatable into the human breast cancer cell system. A significant delay in the productivity of these studies has resulted from relocation of the research laboratory from one institution to another and the delayed transfer of funds from the original site to the new institution. As a result, the project has been extended for an additional year.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2000
- Accession Number
- ADA389502
Entities
People
- James M Cook
Organizations
- University of Illinois at Chicago