Phage Display Breast Carcinoma cDNA Libraries: Isolation of Clones Which Specifically Bind to Membrane Glycoproteins, Mucins, and Endothelial Cell Surface
Abstract
Approved tor public release; distribution unlimited 13. ABSTRACT (Maximum 200 Words) We proposed to identify%, by using a novel phage display technique, carbohydrate-binding proteins (lectins), which may play an important role in progression and metastasis of breast cancer. Using blood- group H-expressing glycoprotein fraction as bait, we observed enrichment of phage clones expressing sequences from galectin-3, a lectin with an affinity with the blood-group substance. Since we obtained a multiple number of different clones encoding this protein, the binding was selective and real. We then constructed additional libraries including MCF-7 human breast carcinoma and EJG bovine capillary endothelial cell libraries. We performed in vitro biopanning experiments using as bait purified fractions of glycoproteins and glycolipids, as well as antibodies against plant lectins, and obtained several dozens of candidate phage clones.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2000
- Accession Number
- ADA389517
Entities
People
- Fumiichiro Yamamoto
Organizations
- Sanford Burnham Prebys Medical Discovery Institute