A Potential Tumor Suppressor Protein: Expression and Function in Human Breast Cancer Cells

Abstract

In a search for genes with a role in hormonal control of cell proliferation, we cloned a novel progestin-induced gene, EDD (previously DD5) that appears to be the human homologue of the hyd tumor suppressor gene of Drosophila melanogaster. By amino acid homology with other known proteins it is likely that the EDD protein is an E3 ubiquitin-protein ligase, enzymes which target one or more key proteins for destruction by ubiquitin- mediated proteolysis. The interaction between EDD and two potential ubiquitination substrates was further characterize. Evidence was obtained suggesting that one of these might be regulated by the proteasome while the other most likely serves to localize EDD in the cell. This was supported by cellular fractionation experiments using HEK293 cells over-expressing EDD.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2000
Accession Number
ADA389536

Entities

People

  • Michelle Callaghan

Organizations

  • Garvan Institute of Medical Research

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Detection
  • Genetic Structures
  • Genetics
  • Microsatellites
  • Mutations
  • Neoplasms
  • Proteins
  • Substrates
  • Suppressors

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Genetics
  • Small Business Innovation Research Program (SBIR) EDI Research and Innovation.