Use of Intraductal Adenovins Transduction to Assess the Mammary Tumorigenic Potential of a Constitutively Active Prolactin Receptor
Abstract
We have made good progress in establishing adenovirus transduction as a method for the short term alteration of gene expression in the mammary epithelium. Thus, detailed conditions for reasonably efficient transduction of the mammary epithelium of the late pregnant mouse have been worked out, transduction with adenovirus and expression of green fluorescent protein, GFP, have been shown not to interfere with the function of the mammary cells in which they are expressed. Transgenic technology has been used to evaluate the effects of an activated prolactin receptor, aPRLR, and an activated member of the prolactin signal transduction pathway, Akt, on the mammary epithelium. The latter has been shown to prolong mammary involution and preliminary results suggest it may enhance tumorigenesis. Some changes in direction are warranted: A functional proof of principle experiment utilizing expression of the cytoplasmic tail of the tight junction protein, ocoludin, is in progress. Adenovirus expressing Akt will be used with a mouse model of tumorigenesis, a mouse overexpressing the neu oncogene, to examine the question of whether adenovirus can be used to express genes that promote tumorigenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2000
- Accession Number
- ADA389838
Entities
People
- Margaret Neville
Organizations
- University of Colorado Health