Tumor-Targeting Peptides for Therapeutic Gene Delivery

Abstract

The identification of markers expressed on specific tumors would give valuable insights into the specialization of tumor vasculature, and would also provide a means of targeting distinct tumor sites. We have recently developed a novel approach for in vivo selection of peptides capable of homing into specific organs or tumors. We have demonstrated the feasibility of the approach by isolating a panel of targeting peptides that bind selectively to receptors either within the angiogenic vasculature of tumors or directly on tumor cells. In this proposal we intend to extend this approach by screening experimental breast carcinoma models in vivo. We will use phage display peptide libraries to identify tumor homing peptide motifs capable of recognizing breast tumors. Using targeting peptides, we have shown in animal experiments that an anthracyclin drug can be converted into a less toxic and more potent anti-cancer agent than the free drug. Here we propose to generate molecular conjugates that can target gene therapy vectors to breast tumors in vivo following intravenous administration. Since only a small subset of tumors are accessible to direct injection, targeted vectors would overcome the current limitations associated with the delivery of therapeutic genes.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2000
Accession Number
ADA389899

Entities

People

  • Renata Pasqualini

Organizations

  • Sanford Burnham Prebys Medical Discovery Institute

Tags

DTIC Thesaurus Topics

  • Antineoplastic Agents
  • Blood
  • Blood Vessels
  • Breast Cancer
  • Cancer
  • Carcinoma
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Gene Delivery
  • Gene Therapy
  • Neoplasms
  • Oncology
  • Peptides
  • Proteins
  • Targeting

Fields of Study

  • Biology
  • Medicine
  • Physics

Readers

  • Molecular and Cellular Biochemistry
  • Molecular and genetic basis of cancer.
  • Oncology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech