Evaluation of Cycloogenase-2 as a Novel Target for Breast Cancer Prevention
Abstract
Cyclooxygenase (Cox) catalyzes both prostaglandin synthesis and the production of mutagens. Cox-2, the inducible form of COX, is expressed in a variety of human cancers but its role in breast cancer has not been established. Our research is designed to test whether Cox-2 is important in the pathogenesis of mammary cancer, using Wnt-l as a model mammary oncogene. Wnt-l transgenic mice exhibit mammary hyperplasia and subsequently develop mammary carcinomas. The role of Cox-2 in mammary tumorigenesis is being tested by generating Wnt-l transgenic mice of the following Cox-2 genotypes: (+/+), (+/-), and (-/-), and then evaluating the incidence of mammary hyperplasia and carcinoma formation in these animals. Thus far breeding programs have been established and Fl mice generated, which when crossed will produce sufficient offspring of the required genotypes. The final crosses are currently in progress. Meanwhile, we have demonstrated upregulation of Cox-2 gene transcription in Wnt-l-expressing cell lines. We are currently using these cell lines to dissect the molecular mechanism by which Wnt-1 activates Cox-2 transcription.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADA389909
Entities
People
- Andrew J. Dannenberg
Organizations
- Weill Cornell Medicine