Novel Technology for Cloning Prostate Cancer Cell Merkers
Abstract
Purpose of project is to clone, isolate and characterize protein markers specifically expressed by advanced stage, androgen-independent prostate cancers. Two types of markers with this specificity are targeted: proteins secreted specifically by advanced stage prostate cancer cells, potentially useful in serum-based assays for these tumors; markers expressed specifically on the surface of these cells, potentially useful as advanced prostate cancer. Work during the period covered included: (1) Incorporation of the powerful new DNA Microarray Technology into the project, and its use to investigate differential gene expression in the LNCaP series of human cell lines, representing different stages of prostate cancer. (2) Studies demonstrating potential utility but insufficient purity of membrane fraction (MF) prepared from these cells; and use of an alternative fractionation scheme, successful only with the most advanced cells (C4-2B) . We plan to complete the studies under (1), to identify genes regulated during prostate cancer progression; and to use cDNA to the C4-2B MF RNA to probe DNA microarrays, to identify genes
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2001
- Accession Number
- ADA390379
Entities
People
- F. C. Bancroft
Organizations
- Mount Sinai Hospital