Genetic Definition and Phenotypic Determinants of Human Ovarian Carcinomas
Abstract
Ovarian cancer is the fourth leading cause of cancer-related death in U.S. women. This program project approaches the ovarian cancer problem by 1) establishing a human ovarian tissue and clinical data base core facility to support the proposed projects and future investigations, 2) identifying genes which are differentially expressed in ovarian cancers and thereby discovering biomarkers for early detection, 3) studying ovarian tumorigenesis in ovarian tissues obtained from germ line BRCA1 mutation carrier to better understand the interaction between mutational inactivation of BRCA1, the cellular caretaker gene and p53, the cellular gatekeeper gene, and 4) developing a genetically defined mouse model of epithelial ovarian cancer. To date, the ovarian tissue core has banked over 100 surgical specimens and provided sufficient resources for the ongoing projects. Representational difference analysis was used to identify 46 genes overexpressed in normal ovarian epithelium and 14 genes overexpressed in ovarian cancer cells. The BRCA1-mutation associated ovarian tissues required to understand the functional interaction between of p53 and BRCA1 have been identified. And, a new viral construct carrying the cre recombinase under the control of the K18 promoter has been tested to establish its ability to mediate recombination in mouse ovarian epithelial cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2000
- Accession Number
- ADA390427
Entities
People
- Beth Karlan
Organizations
- University of California, Los Angeles