Designer T-Cells for Breast Cancer Therapy: Phase I Studies

Abstract

IgTCR-Modified T Cells for Breast Cancer Therapy: Phase I Studies. T cells are capable of penetrating any biologic space and eliminating cells displaying foreign antigens. We have used a chimeric IgTCR molecule to create T cells with antigen-specificity directed against the CEA tumor antigen. In the present study we have examined the toxicity and tolerability of autologous T cells modified with this receptor in CEA+ breast cancer patients. Patients have tolerated the treatment well, and no adverse events could be attributed to the therapy. Preliminary efficacy data suggest that IgTCR-modified T cells may have some anti-tumor activity in vivo, but are limited by poor circulation after infusion. Research aimed at improving circulation have focused on isolating/expanding subpopulations of T cells with a memory cell-like phenotype. These cells enter a resting state when deprived of IL2 rather than a apoptosis pathway. These memory-like cells may persist and circulate much better than standard ex vivo expanded cells. We have also shown that CD28 co-stimulation signals are required to induce proliferation of IgTCR+ T cells. Incorporating antigen-driven T cell proliferation into the activation pathway should allow any T cells that do infiltrate tumor to proliferate rapidly to high numbers. Together, these results suggest that IgTCR-modified autologous T cells may become an important modality for the treatment of metastatic breast cancer.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1999
Accession Number
ADA390706

Entities

People

  • Richard P. Junghans

Organizations

  • Beth Israel Deaconess Medical Center

Tags

DTIC Thesaurus Topics

  • Apoptosis
  • Azo Compounds
  • Biomedical Research
  • Blood
  • Breast Cancer
  • Cell Count
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Colon Cancer
  • Lymphocytes
  • Manufacturing
  • Molecules
  • Neoplasms
  • Standards
  • T Lymphocytes
  • Tumor Cell Line

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • Oncology
  • Oncology (Cancer Research).

Technology Areas

  • Space