Sensitive, Selective Fluorescent Probes for the Detection and Identification of Nucleic Acids

Abstract

The long-term goals of this project are to develop new strategies for the use of oligonucleotides tagged with multiple fluorescent labels as hybridization probes of specific nucleic acid sequences. First, we have designed (and continue to design) new classes of fluorescent labels for DNA. Second, we have developed novel modes of interaction between fluorescent labels, leading to color changes on detection of nucleic acids. Finally, we have developed new methods for increasing specificity of nucleic acid recognition.

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Document Details

Document Type
Technical Report
Publication Date
Mar 31, 2001
Accession Number
ADA390830

Entities

People

  • Eric T. Kool

Organizations

  • University of Rochester

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Acids
  • Aromatic Hydrocarbons
  • Chemical Compounds
  • Chemical Synthesis
  • Chemistry
  • Cyclic Hydrocarbons
  • Detection
  • Fluorophores
  • Hybridization
  • Identification
  • Ligation
  • Nucleic Acids
  • Recognition
  • Scientists
  • Sequences
  • Technology Transfer

Fields of Study

  • Chemistry

Readers

  • Molecular Genetics

Technology Areas

  • Biotechnology