Functional Analysis of C-CAM1 Tumor Suppressor Gene by Targeted Gene Deletions
Abstract
C-CAM is a cell adhesion molecule of the immunoglobulin supergene family. We have recently shown that C-CAM plays critical roles in prostate cancer initiation and progression and that loss of C-CAM is an early event in the development of prostate cancer. Although tumorigenesis studies in mouse xenograft model have suggested the involvement of C-CAM in epithelial cell growth and differentiation, the functional roles of C-CAM in normal prostate development, prostate homeostasis, and prostate tumorigenesis remain unclear. We propose to determine the roles of C-CAM's growth suppressive activity in prostate growth and tumorigenesis by using gene targeting and embryonic stem cell technologies to generate C-CAM knockout mice. We have isolated and sequenced two closely related Ceacam (C-CAM) genes, i.e., Ceacam1 and Ceacam2, from a mouse 129 Sv/Ev library. We have also examined the tissue-specific and embryonic expressions of these mouse Ceacam1 and Ceacam2 genes. Results from this study allow us to design a gene targeting strategy that is specific to Ceacam1 gene and also allow us to perform both straight and conditioned knockout of Ceacam1 gene in parallel.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2001
- Accession Number
- ADA391027
Entities
People
- Sue-hwa Lin
Organizations
- The University of Texas MD Anderson Cancer Center