Tumor Restrictive Gene Therapy for Metastatic Prostate Cancer
Abstract
The purpose of this proposal is to evaluate the ability of a replication-restrictive adenovirus (Ad-OC-Ela) to specifically target and lyse cells of an androgen independent prostate cancer osseous metastasis, which account for a majority of the morbidity and mortality experience by men with prostate cancer. The scope of this project to perform the studies outlined in proposal to prove the hypothesis that conditional replication under the guidance of the osteocalcin promoter can exert a prostate cancer-specific cell kill in well defined pre-clinical models of human androgen independent prostate cancer metastases. More specifically, Specific Aim I seeks to evaluate the specificity of the tumor-restrictive replication of Ad-OC-E 1 a using in vitro assays on prostate and non-prostate cancer cells. The ability of the Ad-OC-Ela to have at least a 100 fold killing differential favoring OC + cell lines (LNCaP, C4-2, PC-3) over OC- cell lines (LOVO, PrSC). Specific Aim II evaluates the growth inhibition of human prostate cancer xenografts attributable to Ad-OC-E 1 a administration, as well as, the tissue distribution and toxicity profile of such injections. The ability to Ad-OC-Ela to completely destroy both androgen-dependent and androgen-independent human prostate cancer cells in xenografts supports the hypothesis being studied.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2000
- Accession Number
- ADA391081
Entities
People
- Thomas A. Gardner
Organizations
- Indiana University