Survival Pathways and Apoptosis of Breast Cancer Cells as Targets for Therapeutic Modulation
Abstract
Th the past year I have assessed the dependence of breast cancer cell survival on the P13 kinase/Akt pathway. The ability of cells to survive under adverse conditions, such as when growth factors are limited or when chemotherapeutics are present, likely contributes to the progression of breast cancer. Using six breast cancer cell lines and one immortal, but phenotypically normal, breast line, I first looked at the ability of each line to survive under serum-free conditions. Next I assayed the effects of two pharmacological inhibitors of key survival signaling pathways on breast cancer cell survival both under serum-free and serum-containing conditions. The results suggest that most breast cancer cell lines rely more heavily on the MEKl/2-ERKl/2 signaling pathway than on the PI3-kinase ->Akt pathway for survival.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2001
- Accession Number
- ADA391167
Entities
People
- Maureen O. Ripple
Organizations
- Dartmouth College