Adipocyte Differentiation: Relationship to Breast Cancer

Abstract

Our results generated during this report period supported the central hypothesis in this grant application. In summary, we have a two-hit hypothesis. First, malignant epithelial cells block the differentiation of surrounding adipose fibroblasts through cytokines. Then, epithelial factors induce aromatase expression in these undifferentiated fibroblasts via switching aromatase gene promoter use from the physiologically used promoter 1.4 to aberrantly activated promoter II. During this grant period, we demonstrated that the transcription factor C/EBPbeta mediates this malignant epithelial cell effect on adipose fibroblasts. The end result is aromatase overexpression and increased local formation of estrogen in breast cancer. In future, we seek to isolate these malignant epithelial cell-derived factors and associated signaling pathways in adipose fibroblasts. We also will determine whether estrogen regulates the formation of antiadipogenic cytokines IL-11 and TNFalpha in malignant epithelial cells.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2001
Accession Number
ADA391324

Entities

People

  • Serdar E. Bulun

Organizations

  • University of Illinois at Chicago

Tags

DTIC Thesaurus Topics

  • Adipose Tissue
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Culture Media
  • Cytokines
  • Diseases And Disorders
  • Epithelial Cells
  • Estrogens
  • Fibroblasts
  • Gene Expression
  • Neoplasms
  • Polymerase Chain Reaction
  • Proteins
  • Transcription Factors

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Biology and Genetics
  • Molecular and Cellular Biology