Prostate Carcinoma Metastasis Tracked with Histochemical Marker Genes
Abstract
This project examines mechanisms by which human prostate carcinoma (PCA) cells undergo metastasis in an athymic nude mouse (male) model system since little attention has been devoted to these events for PCA. This includes PCA CWR22, CWR22R, and CWR21 xenografts adapted to tissue culture. To track tumor cells at the single-cell level and quantitatively, histochemical marker genes have been transfected for resolution as blue-, red-, or black-staining cells. Specific aim I examines the organ specificity of metastatic spread (particularly to bone and liver which escape detection in most animal models). Lacz-tagged CWR22R cells have been isolated and s.c. injected. We routinely observe micrometastases in lung, liver, and bone and specificity of these events further evaluated in an experimental metastasis model. Aim II examines any significance for androgen for metastatic spread; with CWR22R, androgen-relatedness has been observed for both initiation of primary tumors and specificity of metastasis. Aim III evaluates possible interclonal cooperativity by injecting two different PCA cell types tagged with different marker genes. These experiments identify a new model for human prostate carcinoma derived from a primary tumor (therefore, not an already-metatastically selected population from the patient) that mimicks the progression characteristics of the human disease.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2001
- Accession Number
- ADA391333
Entities
People
- Lloyd A. Culp
Organizations
- Case Western Reserve University