The Involvement of Poly(ADP-RIBOSYL)ation in Defense Against Toxic Agents: Molecular Biology Studies

Abstract

We observed a severe dose and time dependent decrease in cell viability,via apoptosis, by JP-8 in a rat lung epithelial cell line (RLE-6TN) and in monoblastoid (U937) cells, characterized by caspase 3 activation, PARP cleavage and pro-apoptotic, cytochrome c released from mitochondria. Jurkat cells transfected with Bcl-xL, demonstrated increased survivability when exposed to JP-8. Exposure of human keratinocytes to JP-8 markedly downregulates the anti-apoptotic members of the Bcl-2 family, and upregulates the pro-apoptotic members.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2001
Accession Number
ADA391337

Entities

People

  • Mark Smulson

Organizations

  • Georgetown University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Apoptosis
  • Biochemistry
  • Carrier Proteins
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Cellular Structures
  • Chemical Synthesis
  • Chemistry
  • Dna Microarrays
  • Epithelial Cells
  • Genetics
  • Lymphocytes
  • Molecular Biology
  • Neoplasms
  • Programmed Cell Death
  • Proteins

Fields of Study

  • Biology
  • Chemistry

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Toxicology/Environmental Toxicology