Malaria Genome Sequencing Project
Abstract
The objectives of this 5-year Cooperative Agreement between TICR and the Malaria Program, NMPC, were to: Specific Aim 1, sequence 3.5 Mb of P. ralciparum genomic DNA; Specific Aim 2, annotate the sequence; Specific Aim 3, release the information to the scientific community. Two additional Specific Aims have been added: Specific Aim 4, sequencing if P. yoeiii to 3x coverage; Specific Aim 5, sequencing of P. vi vax to 3x coverage. To date, we have published the first complete sequence of a malarial chromosome (chromosome 2 (7), completed the random phase sequencing and closed most of the gaps in 3 other large chromosomes totaling 7.2 Mb, and have initiated functional genomics studies using glass slide micorarrays to characterize the expression of genes from chromosome s 2, 3, 10,11,12 and 14 throughout the erythrocytic cycle. We also collaborated in the construction of a two-enzyme optical restriction map of the entire P. falciparum genome (14), and are continuing to further develop the GlimmerM gene finding software developed in year 1. In addition, we have completed sequencing of the rodent malaria P. yoeiii to 5X coverage and this year will begin sequencing of P. vivax to 3x coverage. A subcontract was awarded to the Scripps Research Institute to use proteomics techniques to identify sporozoite proteins in P. faiciparum and P. yoelii sporozoites, leading to the idenfication of over 300 sporozoite proteins. Discussions with the Malaria Program, NMRC aimed at development of a program to use genomics and functional genomics to accelerate vaccine research are in progress.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2001
- Accession Number
- ADA391595
Entities
People
- Malcolm J. Gardner
Organizations
- J. Craig Venter Institute