High Temperature Superconductor RF Probes for Breast Cancer Research
Abstract
For the fourth year there has been a significant improvement of the in vivo spectroscopy study. An improved cell perfusion system was constructed. Using the improved perfusion system the duration of the cell metabolism studies has been extended to longer than 6 days, which is much longer than the previously reported 14 hours. This long and stable study significantly improved the signal-to-noise ratio of the NMR spectrum. Many phosphorus metabolites have been identified including: phosphoethanolamine (PE), phosphocholine (PC), inorganic phosphate (Pi), glycerophosphoethanolamine (GPE), glycerophosphocholine (GPC), phosphocreatine (PCr), gamma-adrinophosphate (gamma-ATP), alpha-adrinophosphate (alpha-ATP), diphosphodiester (DPDE), and beta- adrinophosphate (beta-ATP). Some of the entwined peaks, particularly in the PE/PO, GPE/GPC, and intra- and extra-cellular Pi regions, can be separated. With the improved NMR perfusion system four MCF7 breast cancer cells drug sensitivity studies were conducted. Drugs with different concentrations were perfused through the NMR tube. The concentrations of the high energy phosphates such as ATP and POr decreases and the intracellular Pi increases. The GPE and GPC shows a slight increase initially and a decrease later. Changes in phosphorus metabolic activity indicate that the cells were under stress when first exposed to the drug. Later, the toxic effects of the drug overwhelmed the cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2000
- Accession Number
- ADA391596
Entities
People
- Paul C Wang
Organizations
- Howard University